ABSTRACT
Telfairia occidentalis possesses high antioxidant activity. However, the antioxidant components of the plant have not yet been identified. This study was undertaken to identify the phenolics in the leaf of the plant. Extract and fractions of the leaf of the plant were analysed using the HPLC and GCMS. HPLC analysis revealed the presence of gallic acid [22.19microg/mg], catechin [29.17microg/mg], caffeic acid [9.17microg/mg], ferulic acid [0.94microg/mg], sinapic acid [1.91 microg/mg] and 4-hydroxy benzoic acid [43.86 microg/mg] in the aqueous extract. Phenolics fraction contained gallic acid [0.88 microg/mg], catechin [2.70microg/mg], caffeic acid [7.92microg/mg], ferulic acid [2.72microg/mg], benzoic acid [6.36microg/mg], pcoumaric acid [1.48microg/mg], quercetin [12.00microg/mg]. Only caffeic acid [2.50microg/mg], ferulic acid [0.44microg/mg] and quercetin [8.50microg/mg] were detected in the flavonoid fraction. While GCMS analysis showed the presence of methylparaben; ethylparaben; benzoic acid; 4-hydroxy-2-methoxy-3,5,6-trimethyl-, methyl ester; 4-hydroxy-3-methoxy; phenol, 5-methoxy-2-[methoxymethyl]-; phenol, 5-methoxy-2, 3- dimethyl; and phenol, 2-[2-benzothiazolyl]-. This study is the first to reveal the identity of some phenolics components of the leaf of Telfairia occidentalis
ABSTRACT
Pioglitazone, peroxisome proliferator-activated receptor [PPAR-gamma] agonist, is a therapeutic drug for diabetes. Present study investigated the interaction between PPAR-gamma and alpha adrenoceptors in modulating vasopressor responses to Angiotensin II [Ang II] and adrenergic agonists, in diabetic and non-diabetic Spontaneously Hypertensive Rats [SHRs]. Diabetes was induced with an i.p injection of streptozotocin [40 mg/kg] in two groups [STZ-CON, STZ-PIO], whereas two groups remained non diabetic [ND-CO, ND-PIO]. One diabetic and non-diabetic group received Pioglitazone [10mg/kg] orally for 21 days. On day 28, the animals were anaesthetized with sodium pentobarbitone [60mg/kg] and prepared for measurement of systemic haemodynamics. Basal mean arterial pressure of STZ-CON was higher than NDCON, whereas following pioglitazone treatment, MAP was lower compared to respective controls. MAP responses to i.v administration of NA, PE, ME and ANG II were significantly lower in diabetic SHRs: STZ-CON vs. ND-CON [35%]. Pioglitazone significantly decreased responses to NA, PE, ME and ANG II in ND-PIO versus ND-CON by 63%. Responses to NA and ANG II were significantly attenuated in STZ-PIO vs. ND-PIO [40%]. PPAR-gamma regulates systemic hemodynamic in diabetic model and cross-talk relationship exists between PPAR-gamma and alpha1-adrenoceptors, ANG II in systemic vasculature of SHRs